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Arsenic is highly toxic to human and animals. Previously, we demonstrated that dimeric cysteine crosslinker (CX) dimethyl glutarate is effective in reducing As accumulation in rice seedlings. To evaluate the bioremediation of inorganic As in edible grains, CX was synthesized by reacting cysteine with dimethyl glutarate and was then applied to bread wheat. We found that the content of total As in wheat grains was reduced by 25% by CX treatment for 60 min, even at the As level of 0.5 mg/kg of As in soil. In contrast, Pb and Zn accumulation was not affected by CX treatment. HPLC, LC-MS and GC-MS analyses suggested that glutathione (GSH) in wheat grains was depleted by CX treatment. The As content in CX-treated grains was more efficiently chelated by NaHCO3 than Ca(OH)2. Both As accumulation and GSH reduction by CX were observed only in hulless wheat grains, but not in whole wheat grains. Furthermore, GSH reduction by CX was confirmed at the level of single wheat cells, suggesting its ability to chelate intracellular As at the cellular level. Our results indicate that CX treatment may be effective in reducing As accumulation in edible grains.:12.036: W/System.err(5880): at com.facebook.widget.FacebookDialog.show(FacebookDialog.java:200)
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